Student Faculty of Veterinary Medicine, Gadjah Mada University, Artina Prastiwi, find herbs that can inhibit the growth of the bird flu virus / avian influeza (AI) H5N1. This invention won first prize in the competition Scientists Society and Technology Indonesia (MITI) Paper Challenge (MPC) 2011 which was held last January 29, 2011.Artina find Mahkota Dewa fruit extract (Phaleria macrocarpa) appeared to effectively inhibit the virus that had horrendous world a few years ago. Student generation of 2007 I won thanks to propose a series of studies contained in the scientific paper entitled "Activity Test
Antiviral Extract Infusion Mahkota Dewa (Phaleria macrocarpa) In ovo In Replication Inhibition of H5N1 virus vaccine Organic As Pioneer In Asia ". He managed to set aside 96 other papers from all over Indonesia.
As a vet candidates, Artina admitted restless with the phenomenon of the spread of AI virus in Indonesia who have been eating quite a lot of casualties. He understands very well the risks people would intersect directly with poultry susceptible to this virus.
He is anxious for the fate of the breeders who not only suffered material losses due to this deadly virus attacks, but also the safety of their souls. Although farmers know the risks that arise, they rarely give a vaccine to prevent the AI virus to poultry. "Many ranchers are not memvaksin poultry because of the price of chemical AI vaccine on the market is quite expensive. The price is set at 200 thousand dollars for 100 doses, "he said, quoted by page UGM, Thursday, March 3, 2011.
Starting from the fact that, this scarf girls trying to find solutions to address these troubling issues. He used the fruit crown of god, local potential of Indonesia, which has been scientifically proven to increase endurance as antivirus AI. The content of saponins in the fruit crown gods useful addition to boost the immune system and vitality can also be used as an anti-bacterial and anti-virus.
To get the compound saponin, Artina gods crown fruit extract through distillation. How to make antivirus an extract from the crown of god begins with a weighting according to the dosage required. For a dose of 10 ml dry god crowns needed 100 grams per 100 ml of water or the multiplication of 100 grams per 1000 ml. "Next step is to get the extract distillation," he said at GMU.
After obtaining the extract, Artina do saponin levels in laboratory testing LPPT UGM. He did the test 10 ml of saponin content in LPPT UGM. According to him, extract the crown of the god must contain saponin content of 10 percent. The results obtained saponin is used as a raw material that is as a solvent suspense AI virus antigen. Then used as the vaccine is 0.2 ml extract gods crown.
At first, spoken Artina, tests conducted on 30 embryonated chicken eggs. From the test results are known egg AI virus and given an additional 10 percent of saponin extract of the fruit crown the god of 0.2 ml after incubation for 35 days found no dead embryos, healthy and without scarring. While the eggs are injected with the higher dose 15 percent and 20 percent, turned out all the embryos die with a form of bleeding throughout the body, dwarfism, and alantois turbid fluid.
"Ten percent is the best result to prevent the bird flu virus. This proves that the saponin concentration used should be appropriate because the can cause poisoning if given in large doses, "he said.
Once proven safe in the egg, the couple's daughter and Wartinah Sugita is testing this on chickens aged less than 21 days and the results are quite encouraging. Chickens that have been vaccinated not one death.
It said the girl born in Gunungkidul, January 26, 1989, he developed a vaccine shown to inhibit the development of AI virus to 87 percent. In addition tested in laboratory scale capable of inhibiting AI virus, the vaccine is also cheaper than chemical vaccines sold in the market.
AI vaccine on the market are usually priced 200 thousand rupiah per 100 dose. While vaccine-made 75 thousand dollars per 100 doses. Although somewhat effective and inexpensive, this vaccine has not been mass marketed. "It remains to be done further research, to know for certain results," said alumnus SMA 1 Wonosari this.
The results of this study according to the plan also will dipersentasikan in
AMSTECS International seminar held in Japan on March 19 to 20 next.
* Source: VIVAnews.
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